Fetal Bovine Serum – Sterile. 10 bio-500

STERILE FETAL BOVINE SERUM

Catalog No.: 10 bio-500
Quantity: 500 ml
Storage: -20ºC
Minimum order 10 units

Origin: Brazil

The product is collected from fetuses in an aseptic place, in selected slaughterhouses. The animals are inspected by the Federal Inspection Service of the Ministry of Agriculture (SIF), for export of organotherapy products to the European Economic Community (EEC).
The product is packaged and standardized in 4.5Lt gallons. Each gallon is visually inspected to verify its proper labeling and the integrity of the product and packaging.
Collection, handling, processing and storage are conducted in order to maintain the properties and qualities of the serum. Each batch is accompanied by a certificate containing all the data of the biochemical tests carried out on the sera.

Hemoglobin Levels:
The standard required by the market is less than 25 mg/dl.
Our average is 15 mg/dL.

Collection Method:
They are collected aseptically, by cardiac or venous puncture.

End filter size:
All sera are filtered through three cellulose acetate membranes with a porosity of less than 0.22 m.

Sterility:
All batches of sera are tested for the presence of bacteria, fungi and other possible contaminants.

Mycoplasma:
Each batch is tested for the presence of Mycoplasma in liquid and solid media for 14 days. This parameter is also detected through the Polymerase Chain Reaction, using specific primers for the genus.

Hemoglobin:
The concentration of hemoglobin in the serum is determined by colorimetric method through an assay where the peroxidase activity of hemoglobin is measured using ortho-toluidine as a substrate.

Protein electrophoresis:
Examined for protein pattern characteristics on cellulose acetate tape and pH 8.6 barbital buffer.

Test in Cell Culture:
Sera are tested at concentrations of 5 to 30% in order to subculture 10 generations of subcultures.

Heat inactivation:
The serum is inactivated at 56°C for thirty minutes. This specification is valid for inactivated sera.

Specifications:
Each related serum is tested as a growth promoting supplement for defined media. The choice of whey is related to:

cell lineage type
Type of Culture
Chemical composition of the medium
Objects of investigation or use